Purification of Crude Extract

Gilson is pleased to offer a complete range of centrifugal partition chromatography (CPC) systems. Also known as counter current chromatography (CCC), CPC is a liquid-liquid purification technique that does not require traditional solid supports. The main aim of this silica-free technology is to isolate the maximum amount of a specific molecule at the highest purity, in a short
time, and without using a silica column or any support media.

Key Benefits of CPC Purification

• No column to replace or silica to recycle
• Five times less solvent consumption
• High injection capacity (from milligrams to many kilograms of sample)
• 95% recovery – no irreversible absorption or denaturation of fragile molecule
• 99% purity or better
• Complex sample injection/purification in one step
• Adaptable to diverse application fields from isolation of compounds from complex natural extracts to purification of proteins and synthetic molecules and fermentation broth

Why Choose CPC Systems?

• A variety of configuration options available: all CPC systems can be connected to either a PLC Purification System or Gilson modular HPLC system in place of a traditional LC column

• Entirely automated solution with dedicated Gilson Glider CPC software when configured with a PLC Purification System

• Targeted detection via UV/VIS, ELSD, and MS. Mass-directed purification saves time and money by targeting compounds of interest, which results in a reduction of the overall number of fractions collected that require dry down, reconstitution, and analysis.